diff --git a/src/hotspot/os/windows/os_windows.cpp b/src/hotspot/os/windows/os_windows.cpp
index d00babef40f..2b74cccb072 100644
--- a/src/hotspot/os/windows/os_windows.cpp
+++ b/src/hotspot/os/windows/os_windows.cpp
@@ -6058,6 +6058,11 @@ static inline HANDLE get_thread_handle_for_extended_context(DWORD tid) {
 // Thread sampling implementation
 //
 void SuspendedThreadTask::internal_do_task() {
+#if INCLUDE_JFR
+  assert(NOT_COMPILER2(true) COMPILER2_PRESENT(!HotCodeHeap) ||
+         SuspendedThreadTask_lock->owned_by_self(),
+         "suspend/resume must be serialized when HotCodeHeap is enabled");
+#endif
   const HANDLE h = get_thread_handle_for_extended_context(_thread->osthread()->thread_id());
   if (h == nullptr) {
     return;