diff --git a/src/hotspot/share/opto/vectorIntrinsics.cpp b/src/hotspot/share/opto/vectorIntrinsics.cpp
index cee221c8b75..139dd62dc28 100644
--- a/src/hotspot/share/opto/vectorIntrinsics.cpp
+++ b/src/hotspot/share/opto/vectorIntrinsics.cpp
@@ -1037,8 +1037,20 @@ bool LibraryCallKit::inline_vector_mem_operation(bool is_store) {
 
   const bool needs_cpu_membar = is_mixed_access || is_mismatched_access;
 
-  bool mismatched_ms = from_ms->get_con() && !is_mask && arr_type != nullptr && arr_type->elem()->array_element_basic_type() != elem_bt;
+  // For non-masked mismatched memory segment vector read/write accesses, intrinsification can continue
+  // with unknown backing storage type and compiler can skip inserting explicit reinterpretation IR after
+  // loading from or before storing to backing storage which is mandatory for semantic correctness of
+  // big-endian memory layout.
+  bool mismatched_ms = LITTLE_ENDIAN_ONLY(false)
+      BIG_ENDIAN_ONLY(from_ms->get_con() && !is_mask && arr_type != nullptr &&
+                      arr_type->elem()->array_element_basic_type() != elem_bt);
   BasicType mem_elem_bt = mismatched_ms ? arr_type->elem()->array_element_basic_type() : elem_bt;
+  if (!is_java_primitive(mem_elem_bt)) {
+    if (C->print_intrinsics()) {
+      tty->print_cr("  ** non-primitive array element type");
+    }
+    return false;
+  }
   int mem_num_elem = mismatched_ms ? (num_elem * type2aelembytes(elem_bt)) / type2aelembytes(mem_elem_bt) : num_elem;
   if (arr_type != nullptr && !is_mask && !elem_consistent_with_arr(elem_bt, arr_type, mismatched_ms)) {
     if (C->print_intrinsics()) {
diff --git a/test/hotspot/jtreg/compiler/vectorapi/TestBiMorphicMismatchedMemSegment.java b/test/hotspot/jtreg/compiler/vectorapi/TestBiMorphicMismatchedMemSegment.java
new file mode 100644
index 00000000000..efe1d377454
--- /dev/null
+++ b/test/hotspot/jtreg/compiler/vectorapi/TestBiMorphicMismatchedMemSegment.java
@@ -0,0 +1,67 @@
+/*
+ * Copyright (c) 2024, Oracle and/or its affiliates. All rights reserved.
+ * DO NOT ALTER OR REMOVE COPYRIGHT NOTICES OR THIS FILE HEADER.
+ *
+ * This code is free software; you can redistribute it and/or modify it
+ * under the terms of the GNU General Public License version 2 only, as
+ * published by the Free Software Foundation.
+ *
+ * This code is distributed in the hope that it will be useful, but WITHOUT
+ * ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or
+ * FITNESS FOR A PARTICULAR PURPOSE.  See the GNU General Public License
+ * version 2 for more details (a copy is included in the LICENSE file that
+ * accompanied this code).
+ *
+ * You should have received a copy of the GNU General Public License version
+ * 2 along with this work; if not, write to the Free Software Foundation,
+ * Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA.
+ *
+ * Please contact Oracle, 500 Oracle Parkway, Redwood Shores, CA 94065 USA
+ * or visit www.oracle.com if you need additional information or have any
+ * questions.
+ */
+
+package compiler.vectorapi;
+
+/*
+ * @test
+ * @bug 8329555
+ * @modules jdk.incubator.vector
+ *
+ * @run main/othervm -Xbatch -XX:+TieredCompilation compiler.vectorapi.TestBiMorphicMismatchedMemSegment
+ */
+
+
+import jdk.incubator.vector.ByteVector;
+
+import java.lang.foreign.MemorySegment;
+import java.nio.ByteOrder;
+import java.util.concurrent.ThreadLocalRandom;
+import java.util.concurrent.atomic.AtomicLong;
+import java.util.stream.IntStream;
+
+public class TestBiMorphicMismatchedMemSegment {
+    public static void main(String[] args) {
+        AtomicLong aLong = new AtomicLong();
+
+        IntStream.range(0, 10000).forEach(j -> {
+            byte[] bytes = new byte[64];
+            ThreadLocalRandom.current().nextBytes(bytes);
+            var byteSegment = MemorySegment.ofArray(bytes);
+            var byteFragment = ByteVector.SPECIES_PREFERRED.fromMemorySegment(byteSegment, 0, ByteOrder.LITTLE_ENDIAN);
+            float[] floats = new float[128];
+            byte[] targetBytes = new byte[512];
+            var floatSegment = MemorySegment.ofArray(floats);
+            var targetByteSegment = MemorySegment.ofArray(targetBytes);
+            byteFragment.intoMemorySegment(floatSegment, ThreadLocalRandom.current().nextInt(0, 448), ByteOrder.LITTLE_ENDIAN);
+            byteFragment.intoMemorySegment(targetByteSegment, ThreadLocalRandom.current().nextInt(0, 448), ByteOrder.LITTLE_ENDIAN);
+            var l = 0;
+            for (int i = 0; i < floats.length; i++) {
+                l += (int) floats[i];
+            }
+            aLong.addAndGet(l);
+        });
+
+        System.out.println(aLong.get());
+    }
+}